The laccase yield obtained from the 18 sets of fermentation experiments performed with the selected factors and levels was further processed with Qualitek-4 software. glucose, yeast extract, malt extract, inoculum, mineral solution, inducer (1 mM CuSO4) and amino acid (l-asparagine) at three levels and pH at two levels, with an OA layout of L18 (21 × 37) were selected for the proposed experimental design. This methodology optimizes the number of impact factors and enables to calculate their interaction in the production of industrial enzymes. This approach facilitates the study of the interactions of a large number of variables spanned by factors and their settings, with a small number of experiments, leading to considerable savings in time and cost for process optimization. Production of laccase using a submerged culture of Pleurotus orstreatus IMI 395545 was optimized by the Taguchi orthogonal array (OA) design of experiments (DOE) methodology.
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